In this paper, we present high- and middle-resolution spectral observations of seven heartbeat (HB), highly eccentric, Kepler binaries: four of them (KIC 3230227, KIC 03547874, KIC 03749404, KIC 07672068) are SB2 stars and the remaining three (KIC 04949194, KIC 05960989, KIC 10092506) are SB1 stars. The flux amplitudes of their HB signals are from 0.001 to 0.01. Five targets of the sample with eccentricity above 0.64 show tidally induced oscillations, which are harmonics of the orbital period. The orbital and global parameters of all targets were determined as a result of simultaneous modelling of our spectral observations and Kepler photometric data. They exhibit that the masses and radii of some target components do not obey the temperature-mass-radius relation of main-sequence stars. Although our targets do not obey precisely the theoretical period-eccentricity relation, they confirm the previous conclusion that HB stars draw the upper envelope of the eccentricity-period distribution. We did not find simple empirical dependences of the amplitude of the HB signal on the orbital and global parameters of the targets.