Three hundred and eighty eight hot subdwarf stars have been identified by using the Hertzsprung-Russell (HR) diagram built from the second data release of the Gaia mission. By analyzing their observed Large Sky Area Multi-Object Fibre Spectroscopic Telescope (LAMOST) spectra, we characterized 186 sdB, 73 He-sdOB, 65 sdOB, 45 sdO, 12 He-sdO, and 7 He-sdB stars. The atmospheric parameters of these stars (e.g., Teff, logg, log(nHe/nH)) are obtained by fitting the hydrogen (H) and helium (He) line profiles with synthetic spectra calculated from non-local thermodynamic equilibrium model atmospheres. Among these stars, we have 135 new identified hot subdwarfs which have not been cataloged before. Although 253 stars appear in the catalog by Geier+ (2017, J/A+A/600/A50), only 91 of them have atmospheric parameters. Together with the 294 hot subdwarf stars found by Lei+ (Paper I, 2018, J/ApJ/868/70), we identified 682 hot subdwarf stars in total by using the Gaia HR-diagram and LAMOST spectra. These results demonstrate the efficiency of our method to combine large surveys to search for hot subdwarf stars. We found a distinct gap in our He-sdOB stars based on their He abundance, which is also presented in extreme horizontal branch (EHB) stars of the globular cluster {omega} Cen. The number fraction of the sample size for the two subgroups is very different between the two counterparts. However, the distinct gap between the H-sdB stars and He-sdOB stars in {omega} Cen is not visible in our sample. More interestingly, the He-sdB population with the highest He abundance in our sample is completely missing in {omega} Cen. The discrepancy between our field hot subdwarf stars and the EHB stars in {omega} Cen indicate different origins for the two counterparts.