We present a comprehensive study of the total X-ray emission from the colliding galaxy pair NGC 2207/IC 2163, based on Chandra, Spitzer, and GALEX data. We detect 28 ultraluminous X-ray sources (ULXs), 7 of which were not detected previously because of X-ray variability. Twelve sources show significant long-term variability, with no correlated spectral changes. Seven sources are transient candidates. One ULX coincides with an extremely blue star cluster (B-V=-0.7). We confirm that the global relation between the number and luminosity of ULXs and the integrated star-formation rate (SFR) of the host galaxy also holds on local scales. We investigate the effects of dust extinction and age on the X-ray binary (XRB) population on subgalactic scales. The distributions of N_X_ and L_X_ are peaked at L_IR_/L_NUV_~1, which may be associated with an age of ~10 Myr for the underlying stellar population. We find that approximately one-third of the XRBs are located in close proximity to young star complexes. The luminosity function of the XRBs is consistent with that typical for high-mass XRBs and appears unaffected by variability. We disentangle and compare the X-ray diffuse spectrum with that of the bright XRBs. The hot interstellar medium dominates the diffuse X-ray emission at E<~1 keV and has a temperature kT=0.28_-0.04_^+0.05^ keV and intrinsic 0.5-2 keV luminosity of 7.9x10^40^ erg/s, a factor of ~2.3 higher than the average thermal luminosity produced per unit SFR in local star-forming galaxies. The total X-ray output of NGC 2207/IC 2163 is 1.5x10^41^ erg/s, and the corresponding total integrated SFR is 23.7 M_{sun}_/yr.