Globular clusters (GCs) are among the oldest stellar systems in the universe. As such, GCs population are valuable fossil tracers of galaxy formation and interaction history. This paper is part of the VEGAS-SSS series, which focuses on studying the properties of small stellar systems (SSSs) in and around bright galaxies. We use the multi-band wide-field images obtained with the VST to study the properties of the globular cluster (GC) population in an interacting pair of galaxies. We derived ugri photometry over 1.5x1.5 sq. degrees centred on the galaxy group composed by two elliptical galaxies: NGC 3640 and its fainter companion NGC 3641. We studied the GC system properties from both the ugri and gri matched catalogs. GC candidates were identified based on a combination of photometric properties (colors, magnitudes) and morphometric criteria (concentration index, elongation, FWHM, etc.), using sources with well-defined classifications from spectroscopic or imaging data available in the literature and numerical simulations as references. The selection criteria were also applied to empty fields to determine a statistical background correction for the number of identified GC candidates. The 2D density maps of GCs appear to align with the diffuse light patches resulting from merging events of the galaxies. The highest density peak of GCs is observed to be on NGC 3641 rather than NGC 3640, despite the latter being the more massive galaxy. The azimuthal averaged radial density profiles in both galaxies reveal that the GC population extends beyond the galaxy light profile and indicate the likely presence of an intra-group GC component. A color bimodality in (u-r) and (g-i) is observed for NGC 3641, whereas NGC 3640 shows a broad unimodal distribution. Analysis of the GC Luminosity Function indicates that both galaxies are roughly located at the same distance (~27Mpc). We provide an estimate of the total number of GCs, and determine the specific frequency for NGC 3640, S_N_=2.0+/-0.6, which aligns with expectations, while for NGC 3641 we find a large S_N_=4.5+/-1.6.