Deuterium fractionation has been used to study the thermal history of prestellar environments. Their formation pathways trace different regions of the disk and may shed light into the physical structure of the disk, including locations of important features for planetary formation. We aim to constrain the radial extent of the main deuterated species; we are particularly interested in spatially characterizing the high and low temperature pathways for enhancing deuteration of these species. We observed the disk surrounding the Herbig Ae star HD 163296 using ALMA in Band 6 and obtained resolved spectral imaging data of DCO^+^ (J=3-2), DCN (J=3-2) and N_2_D^+^ (J=3-2) with synthesized beam sizes of 0.53"x0.42", 0.53"x0.42", and 0.50"x0.39", respectively. We adopted a physical model of the disk from the literature and use the 3D radiative transfer code LIME to estimate an excitation temperature profile for our detected lines. We modeled the radial emission profiles of DCO^+^, DCN, and N_2_D^+^, assuming their emission is optically thin, using a parametric model of their abundances and our excitation temperature estimates.