We present a second update of the Arcetri Catalog of water masers (Comoretto et al., 1990A&AS...84..179C; Brand et al., 1994, Cat. <J/A+AS/103/541>). The present study reports the results of the observations carried out with the Medicina 32-m radiotelescope from January 1993 to April 2000 on a sample of 300 sources. This compilation consists of newly discovered maser sources that did not appear in the previous Arcetri Catalogs and is made of: a) detections from the literature, and b) unpublished detections obtained with the Medicina antenna. Overall, 83 out of 300 sources were detected. The detection rate is low (28%) and we attribute this result to the inclusion in our survey of a rather large number of spurious maser detections that have appeared in one particular paper. The observational parameters are reported in tabular form for all the 300 sources and the spectra of the detected masers are presented. We discuss the global properties of the complete Arcetri Catalog based on Comoretto et al. (1990A&AS...84..179C), Brand et al. (1994, Cat. <J/A+AS/103/541>) and the present observations, which now contains 1013 galactic water maser sources. Of these, 937 have an IRAS counterpart within 1 arcmin from the nominal position of the maser. We establish a classification scheme based on the IRAS flux densities which allows to distinguish between water masers associated with star forming regions and late-type stars. The Arcetri Catalog represents a useful data base for systematic studies of galactic water maser sources.