Based on a preliminary reference catalogue, combined with the catalogues PPM, ACRS and those in Eichhorn et al (1970, Cat. <I/90>), Jones (1973A&AS....9..313J) and Hertzsprung (1947AnLei..19a...1H), 33 exposures on 11 plates taken with the Zo-Se 40cm refractor (f=6895mm) in 86 years are reduced with the central overlapping technique, and high-precision positions and proper motions of 441 stars in the Pleiades astrometric standard region are obtained. The standard errors of star positions are less than +/-0.05arcsec, and the standard errors of proper motions for 90% stars in our reduction are less than +/-0.001arcsec/yr.